Session Index

We have developed a long wavelength optical coherence tomography imaging system allowing volumetric imaging of the ex vivo mouse brain and cerebellum tissue architectures. Objectives with two different magnifications (5X and 10X) were mounted to the power turret allowing multiscale imaging of the brain and cerebellum tissue.

 

Optical trapping has been widely used in a variety of research fields for manipulating micro/nano meter size particles. Here, we are demonstrating holographic optical tweezer technique using circular Airy beams for trapping of micron size particles. Present results may find important applications in the field of biophotonics and related fields.

 

Applying traction force microscopy in conjunction with rectangular micropattern to investigate the nuclear stiffness of metastatic vs. non-metastatic tumor cells, we observed that the nucleus of metastatic tumor cells was softer than that of non-metastatic tumor cells.

 

Atomic force microscope (AFM) has unique capabilities to measure multiple surface properties. However, the low imaging rate and the surface-limited measuring range of AFM limit its application in cell imaging. Herein, we propose a hybrid system of an AFM and a total internal reflection fluorescence microscope to overcome the limitations.

 

The problems of the treatment of attention deficit hyperactivity disorder (ADHD) contains the side effects in drug use, and the complicated process of behavioral therapy. In this study, we believe that our treatment can improve the condition from the feedback of collected 5 ADHD patients for 8 weeks of treatment.

 

The hybrid photodetector provides different current level according to different number of detected signal photons. This superior feature is adopted for the advanced optoelectronic signal detection for dynamic range improvement of single photon counting technique for fluorescence detection which is widely used for the point scanning-based multiphoton and confocal microscopy.

 

Differential phase contrast (DPC) microscopy is used to reconstruct phase image of transparent specimen. We propose a novel illumination method to achieve isotropic differential phase contrast (iDPC) and improve the accuracy and stability of phase recovery. We also implemented the Wavelength-Coded illumination pattern to shorten image acquisition time.

 

In this study, dynamic color temperature light can improve sleep quality which is very similar to the dark environment and is superior to static light sources. In terms of work efficiency, the test results of dynamic color temperature light and static light source are significantly better than the dark environment.

 

In this work, digital micro-mirror device (DMD) based optical sectioning micro-endoscopy using multi-mode fibers attached with Gradient-index (GRIN) lens is presented. The proposed system utilizes DMD for structured illumination to acquire three dimensions (3D) optical sectioning images of a volumetric sample in high speed fashion.

 

In this paper, flatness splitting ratio responses of the traditional directional coupler (DC) and Mach-Zehnder directional coupler (MZDC) to optical coherence tomography (OCT) on chip are proposed and demonstrated. The wavelength flatness of these two types of couplers is analyzed and MZDC could improve the signal-to-noise ratio up to 7-dB.

 

In this study, we have developed an SS-OCT system with a 200 kHz, 1060 nm wavelength source, and a GPU card to achieve a real time OCT imaging processing framework. An approximately 1000-fold improvement in processing speed can be observed while GPU is used for OCT data processing in general.

 

Though the cost and configuration of the spectral-domain optical coherence tomography can be decreased by the use of USB 3.0 linescan camera, a precise synchronization between the data acquisition and scanning beam remains challenging. In this study, we use a single-board programmable microcontroller (Arduino) to address the above synchronization issue.

 

There is no scientific mechanism for postoperative evaluation of peripheral arterial occlusive disease (PAOD) currently. Therefore, this study applied functional near-infrared spectroscopy (NIRS) to patients with PAOD, also used far-infrared irradiation as a physiological intervention to evaluate the blood circulation. The results show the feasibility of this method.

 

Despite no real-level transition required in third harmonic generation (THG) process, THG is known being able to be enhanced though real-level absorption. Suppressing the absorption through ground-state depletion, the THG intensity can be modulated for the purpose of improving the resolution of THG microscopy.

 

The laundry detergent for clothes cleaning is a daily necessity in the modern life. Residual concentration of detergent in the washing clothes might affect an allergic reaction of sensitivity skin. This study provided a spectrometric method to analyze the transmission spectrum of the commercial laundry detergent with various concentrations.

 

The theoretical field of view (FOV) of single photon emission microscope is first derived from imager geometry. The distributions of sampling completeness coefficient (SCC) are also simulated to find the FOVs of circular and helical trajectories. The comparison of FOVs from both methods justifies the FOV estimation by SCC simulations.

 

We demonstrate an in vivo high-speed scanning multiphoton microscopy system which is designed for neuroimaging. The system can provide non-invasive 3D video with more than 30 volumes per second for capturing dynamic target. In addition, a sharp 3D scan mode via optical slice reconstruct tissue structure is offered.

 

At high excitation intensity, enhanced green fluorescent protein (eGFP) sample selectively shows over-saturation on fluorescence emission because of different concentrations, which we call selectively over-saturated fluorescence emission (SOFE). In this paper, we investigate the two-photon SOFE microscopy to improve the image resolution down to the order of 30nm.

 

A cost-efficient sensor embedded in washing machine is a key-component for evaluating the residual concentration of detergent. In this study, an alternative sensor was proposed and demonstrated an optimum resolution in concentration of 0.001% (v/v).

 

Receptive field is an important hyperparameter in a convolutional neural network (CNN). In this research, we used a CNN model to classify mice skin lesions in Full-Field Optical Coherence Tomography (FF-OCT) images. We further improved the performance by selecting the receptive fields based on the domain knowledge of skin tissue.